Visualization of nucleic acids

Cover of: Visualization of nucleic acids |

Published by CRC Press in Boca Raton .

Written in English

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Subjects:

  • Nucleic acids -- Analysis.,
  • Nucleic acid probes.,
  • Nucleic acid hybridization.

Edition Notes

Includes bibliographical references and index.

Book details

StatementGerard Morel, [editor].
ContributionsMorel, Gérard.
Classifications
LC ClassificationsQP620 .V57 1995
The Physical Object
Pagination369 p. :
Number of Pages369
ID Numbers
Open LibraryOL1097532M
ISBN 100849347815
LC Control Number94022190

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This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions.

Spreading methods provide useful details on structural feat. ISBN: OCLC Number: Description: p.: ill. (some col.) ; 26 cm. Contents: Ch. The Classic Methods of Nucleic Acid Visualization for Light Microscopy / Jean-Marie Exbrayat --Ch.

g Nucleic Acids with Scanning Probe Microscopes / Wolfgang M. Heckl and Andreas Engel --Ch. Spreading of Nucleic Acids / Etienne Delain and Eric Le Cam --Ch.

This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic Visualization of nucleic acids book can be seen at atomic-scale : Gerard Morel. The web server issue of Nucleic Acids Research is the 17th in a series of annual issues dedicated to web-based software resources for analysis and visualization of molecular biology data.

View the issue. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy (Methods in Visualization) - Kindle edition by Ronot, Xavier, Usson, Yves. Download it once and read it on your Kindle device, PC, phones or tablets.

Use features like bookmarks, note taking and highlighting while reading Imaging of Nucleic Acids and Quantitation in Photonic Microscopy (Methods in Visualization).Manufacturer: CRC Press. Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms.

Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA : Hardcover.

Functional Organization of the Nucleus: A Laboratory Guide. Edited by Barbara A. Hamkalo, Sarah C.R. Elgin. Part I. Visualization of Nucleic Acids. select article Chapter 5 Nucleic Acid Sequence Localization by Electron Microscopic in Situ Hybridization.

The analysis and visualization of nucleic acids (RNA and DNA) is playing an increasingly important role due to their fundamental importance for all forms of life and the growing number of known 3D.

Illumination of the agarose gels with nm UV light is subsequently used for visualizing the stained nucleic acids. Throughout this chapter, the common methods for staining and visualization of. This book provides tables of Protein Data Bank and Nucleic Acid Database identification codes, so that the reader can easily access and display individual structures, either by direct access to the PDB, NDB, or the EBI and the extensive visualization tools available in them, or by downloading coordinates from the PDB/NDB/EBI and inputting them.

Typically, probing nucleic-acid samples within a gel is unsuccessful because as the DNA probe soaks into a gel, the sample nucleic acids within the gel diffuse out.

Thus, blotting techniques are commonly used to transfer nucleic acids to a thin, positively charged membrane made of nitrocellulose or nylon. Protein structure visualization tools-RASMOL 1. Department of Zoology, GACW () Protein structure visualization tools Introduction: Before computer visualization software was developed, molecular structures were presented by physical models of metal wires, rods and spheres.

Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization.

This book provides insight into several classic techniques, histologica. The structure of nucleic acids consists of a sequence of are four types of nucleotides distinguished by which of the four nucleobases they contain: in DNA these are adenine (A), cytosine (C), guanine (G), and thymine (T).

Nucleic acids have the property that two molecules will bind to each other to form a double helix only if the two sequences are complementary, that is, they. Engineered probes to adapt new photochemical properties upon recognition of target nucleic acids offer powerful tools to DNA and RNA visualization technologies.

Herein, we describe a rapid and effective visualization method of nucleic acids in both fixed and living cells with hybridization-sensitive fluorescent oligonucleotide probes. TopoICE-X (Topological Interactive Construction Engine-Crossing change) is a 3D visualization and manipulation program within KnotPlot (30) that can be used to test various models of topoisomerase action on knots belonging to the rational family of knots.

All. In a two column format, the author investigates the classic techniques employed in the visualization of nucleic acids by light microscopy. The book discusses the chemical structure of nucleic acids, general principles of staining, nuclear dyes, and histochemical methods.

The advent of molecular cloning has enabled the isolation and characterization of individual genes from eukaryotic cells. Understanding the role of genes within cells, however, requires analysis of the intracellular organization and expression of individual genes and their encoded proteins.

In this section, the basic procedures currently available for detection of specific nucleic acids and. This unit is the first in a series of four units covering the analysis of nucleic acid structure by molecular modeling. This unit provides an overview of computer simulation of nucleic acids.

Topics include the static structure model, computational graphics and energy models, generation of an initial model, and characterization of the overall. In order to visualize nucleic acid molecules in agarose gels, ethidium bromide or SYBR Green are commonly used dyes. Illumination of the agarose gels with nm UV light is subsequently used for visualizing the stained nucleic acids.

Throughout this chapter, the common methods for staining and visualization of DNA are described in details. The samples containing analyzed nucleic acids are loaded in the wells formed in one end of the gel.

After the electrical field is applied, __ charged nucleic acid molecules start to move through the gel pores from cathode toward anode. "Genome Visualization by Classic Methods in Light Microscopy covers the classic techniques used to visualize nucleic acids with the use of light microscopy.

The book discusses the chemical structure of nucleic acids, general principles of staining, nuclear dyes, and histochemical methods. This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA.

Attention is mainly paid to fluorescent probes for fluorescence microscopy imaging. Requirements for the target-binding part and the fluorophore making up the probe are. This volume covers the structures, properties, and functions of G-quadruplexes in a wide range of biological disciplines, including therapeutic intervention and biomaterial application.

The chapters explore a wide range of vital and new experimental techniques used in the study of G-quadruplexes.

Computer graphics as a technique for the visualization and manipulation of molecular structures on a graphical display device is introduced. The stepwise representations of molecular structures from character string to 2D and to 3D are discussed.

The character string of SMILES and sequence formats for proteins and nucleic acids are given. As a result, in most situations, the application of in vivo hybridization methods is currently limited to the visualization of highly repetitive DNA sequences or abundant RNA species.

We describe a protocol that enables the visualization and tracking of telomeres in living cells by hybridization with a fluorescent peptide nucleic acid (PNA) probe. Nucleic Acid Structure. Protein Structure. The Central Dogma. Physico‐Chemical Properties of the Amino Acids and their Importance in Protein Folding.

Visualization of Amino Acid Properties Using Principal Component Analysis. Clustering Amino Acids According to their Properties. Summary. References. Self‐Test. Cell-to-cell spread of tobacco mosaic virus (TMV) is presumed to occur through plant intercellular connections, the plasmodesmata.

Viral movement is an active process mediated by a specific virus-encoded P30 protein. P30 has at least two functions, to cooperatively bind single-stranded nucleic acids and to increase plasmodesmatal permeability.

Similar Items. Progress in nucleic acid research and molecular biology. Published: () Modified Nucleic Acids / Published: () Circulating nucleic acids in plasma and serum proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held on November in Hong Kong / Published: ().

CD spectrum, is very sensitive to any structural changes of the macromolecule. So, any interaction of protein with nucleic acids can be studied by observing changes in the CD spectra.

The transitions between double-stranded and single- stranded nucleic acids can be studied with CD spectrophotometer. Cell Biology: Technique #   Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry.

The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt.

Nucleic Acids. DNA Structure and Replication. Nature of Science: Making careful observations—Rosalind Franklin’s X-ray diffraction provided crucial evidence that DNA is a double helix.() β - Skill: Analysis of results of the Hershey and Chase experiment providing evidence that DNA is the genetic material.

Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization.

This book provides insight into several classic techniques, histological as well as histochemical, that can be used. [Bert15] Bertrand, D., et al. () Patient-specific driver gene prediction and risk assessment through integrated network analysis of cancer omics profiles. Nucleic acids research gku The Geometry of the Structure of Nucleic Acids With Regard to the Higher Dimension of the Components: /ch Using three-dimensional visualization of nucleic acid molecules, obtained in the previous chapter, an analysis of the geometry of nucleic acid molecules in.

It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.

Techniques in Molecular Biology (to study the function of genes) Analysis of nucleic acids: Polymerase chain reaction (PCR) Analysis of nucleic acids. DNA molecules can be chemically synthesized Visualization of DNA/RNA samples separated by gel electrophoresis.

Amino acids are represented by three-letter and one-letter abbreviations. Writing the full names of the amino acids is inconvenient, especially for polypeptide chains with many amino acids. Two systems of abbrevia-tions listed in Table offer more convenient methods for representing amino acids.

() Nucleic Acids Research DD doi: /nar/gkw OneDep: Unified wwPDB System for Deposition, Biocuration, and Validation of Macromolecular Structures in the Protein Data Bank (PDB) Archive () Structure doi: / Free, interactive visualization tutorials MVM Molecular Visualization Program and GUI of ZMM.

MVM is a free molecular viewer that can be used to display protein, nucleic acids, oligosacharides, small and macromolecules. It has an intuitive interface.

Molecular Graphics Essentials 3 Molecular Graphics Essentials 1. Introduction M OLECULAR GRAPHICSis the discipline and philosophy of studying mole- cules and their properties through graphical representation1.

PyMOL is an open-source visualization software with 3D rendering capabilities.T1 - Visualization of DNA and protein-DNA complexes with atomic force microscopy. AU - Lyubchenko, Yuri L. AU - Gall, Alexander A. AU - Shlyakhtenko, Luda S.

PY - Y1 - N2 - This article describes sample preparation techniques for AFM imaging of DNA and protein-DNA complexes.While there are thousands of antibodies available to specifically identify viral and cellular proteins, and sufficient fluorescent tags to allow co-visualization of multiple proteins in a single sample, these imaging approaches are often incompatible with conventional methods for visualization of nucleic acids (e.g., hybridization of.

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